Publications
2010
P.J. Ylander, P. Hänninen. Modelling of multi-component immunoassay kinetics - A new node-based method for simulation of complex assays. Biophys. Chem. 151 (2010) 105-110
H. Härmä, G. Sarrail, J. Kirjavainen, E. Martikkala, I. Hemmilä, P. Hänninen. Comparison of homogeneous single-label fluorometric binding assays: Fluorescence polarization and multiparameter QRET technique. Analytical chemistry, 2010.
H. Härmä, S. Laakso, S. Pihlasalo, P. Hänninen, Bertrand Faure, Subhasis Rana, L. Bergström,. Rapid detection of trace amounts of surfactants using nanoparticles in fluorometric assays. Nanoscale, 2010, 2, 69.
H. Härmä, S. Laakso, S. Pihlasalo, P. Hänninen. A fluorometric fullerenol sensor for rapid detection of ionic and non-ionic surfactants. Tenside Surfactants Detergents, 2010.
A. Rozwandowicz-Jansen, J. Laurila, E. Martikkala, H. Frang, I. Hemmilä, M. Scheinin, P. Hänninen, H. Härmä. Homogeneous GTP binding assay for activation of G-protein coupled receptor. Journal of Biomolecular Screening, 2010
2009
Teppo Stenholm, J. V., Pekka Hänninen, Jaana Vuopio-Varkila, Pentti Huovinen, Antti J. Hakanen, Pirkko Kotilainen. MRSA screening by on-line Immunometric monitoring of bacterial growth under selective pressure. Accepted, 2009.
Chaudhary A, Raina M, Harma H, Hänninen P, McShane MJ, Srivastava R. Evaluation of glucose sensitive affinity binding assay entrapped in fluorescent dissolved-core alginate microspheres. Biotechnol Bioeng. 2009
Näreoja T, Määttänen A, Peltonen J, Hänninen PE, Härmä H. Impact of surface defects and denaturation of capture surface proteins on nonspecific binding in immunoassays using antibody-coated polystyrene nanoparticle labels. J Immunol Methods. 2009 Aug 15;347(1-2):24-30.
Martikkala E, Lehmusto M, Lilja M, Rozwandowicz-Jansen A, Lunden J, Tomohiro T, Hänninen P, Petäjä-Repo U, Härmä H. Cell-based beta2-adrenergic receptor-ligand binding assay using synthesized europium- labeled ligands and time-resolved fluorescence. Anal Biochem. 2009 Sep 15;392(2):103-9.
Härmä H, Rozwandowicz-Jansen A, Martikkala E, Frang H, Hemmilä I, Sahlberg N, Fey V, Perälä M, Hänninen P. A New Simple Cell-Based Homogeneous Time-Resolved Fluorescence QRET Technique for Receptor-Ligand Interaction Screening. J Biomol Screen. 2009
S. Tang, M. Moayeri, Z. Chen, H. Härmä, J. Zhao, R.H. Purcell, S.H. Leppla, I.K. Hewlett. Detection of Anthrax Toxin by an Ultrasensitive Immunoassay Using Europium Nanoparticles. Clinical and Vaccine Immunology, 2009, 16, 408.
H. Härmä, R. Suhonen, T. Kololuoma, A. Kärkkäinen, M. Hara, P. Hänninen. Thin Solid Europium(III) Dye Layers as Donors in Time-Resolved Fluorescence Resonance Energy Transfer Assays, Applied Surface Science, 2009, 255, 6529.
S. Pihlasalo, J. Kirjavainen, P. Hänninen, H. Härmä, Ultrasensitive Protein Concentration Measurement Based on Particle Adsorption and Fluorescence Quenching, Anal. Chem. 2009, 81, 4995-5000.
T. Näreoja, M. Vehniäinen, U. Lamminmäki, P. E. Hänninen and H. Härmä. Study on nonspecificity of an immuoassay using Eu-doped polystyrene nanoparticle labels. J. Immunol. Methods 2009, 345 (1-2), 80-89.
Valanne A, Suojanen J, Peltonen J, Soukka T, Hänninen P, Härmä H. Multiple sized europium(III) chelate-dyed polystyrene particles as donors in FRET - an application for sensitive protein quantification utilizing competitive adsorption.Analyst. 2009 May;134(5):980-6A
S. Pihlasalo, M. Hara, P. Hänninen, J. P. Slotte, J. Peltonen, H. Härmä, Liposome-based homogeneous luminescence resonance energy transfer, Anal. Biochem. 2009, 384, 231-237.
2008
Härmä H, Dähne L, Pihlasalo S, Suojanen J, Peltonen J, Hänninen P. Sensitive quantitative protein concentration method using luminescent resonance energy transfer on a layer-by-layer europium(III) chelate particle sensor. Anal Chem. 2008 Dec 15;80(24):9781-6
P. Huhtinen, M. Kivelä, T. Soukka, H. Tenhu, T. Lövgren, H. Härmä. Preparation, characterisation and application of europium(III) chelate-dyed polystyrene–acrylic acid nanoparticle labels. Anal. Chim. Acta, 2008, 630, 211–216.
Härmä H, Graf C, Hänninen P. Synthesis and characterization of core-shell europium(III)-silica nanoparticles. J. Nanopart. Res. 2008, 10, 1221–1224.
M. Västilä, S. Peltonen, E. Alban, J. Soukka, J.T. Soini and U. Ruotsalainen, Application of robust estimators to fluorescence assays, manuscript, submitted to EURASIP Journal on Advances in Signal Processing, 1/2007, In Press Volume 2008, Article ID 170497, 10 pages doi:10.1155/2008/170497
Pekka Hänninen, Juhani T. Soini, et al., Two-photon excitation fluorescence bioassays, Annals of the New York Academy of Sciences 1130:320-326 (2008).
2007
K. Blomstedt, T. Setälä, A. T. Friberg, Arbitrarily short coherence length in wave fields within finite lossless source regions, Phys. Rev. E 75, 026610 (2007)
K. Blomstedt, T. Setälä, A. T. Friberg, Effective degree of coherence: general theory and application to electromagnetic fields, J. Opt. A: Pure Appl. Opt. 9, 907-919 (2007)
Härmä H, Soukka T, Shavel A, Gaponik N, Weller H. Luminescent energy transfer between cadmium telluride nanoparticle and lanthanide(III) chelate in competitive bioaffinity assays of biotin and estradiol. Analytica Chimica Acta 2007, 604, 177.
Hakola L, Eiroma K, Heilmann J, Linna H, Kololuoma T, Härmä H. Inkjet printing as a fabrication method for hydrophobic surfaces. Proceedings of 23rd International Conference on Digital Printing Technologies, 2007.
Roope J. Huttunen, Tomás C. O'Riordan, Pirkko L. Härkönen, Juhani T. Soini, Niko J. Meltola, Pekka E. Hänninen, Aleksi E. Soini Quantitative detection of cell surface protein expression by time-resolved fluorometry, Luminescence (2007)22(3):163-170.
Härmä H, Keränen A-M, Lövgren T. Synthesis and characterization of europium(III) nanoparticle for time-resolved fluoroimmunoassay of prostate-specific antigen. Nanotechnology, 2007, 18.
Koskinen JO, Vainionpää R, Meltola N, Soukka J, Hänninen PE, Soini AE Rapid Method for Detection fo Influenza A and B Virus Antigens by Use of a Two-Photon Excitation Assay Technique and Dry-Chemistry Reagents. Journal of Clinical Microbiology, 45, 11
De_Windt A, Reijo Laaksonen et al. Gene Set Enrichment Analyses Revealed Several Upregulated Pathways in Niemann-Pick Disease Type C Fibroblasts DNA Cell Biol. 2007 Sep;26(9):665-71. PMID: 17683244
Aloys deWindt, Myriam Rai, Lise Bernier, Karin M Thelen, Juhani Soini, Chantal Lefebvre, Satyan Chintawar, Jacques Lavigne, Lilli Nurmi, Leena Kytömäki, Jon Scott Munzer, Dieter Lütjohann, Massimo Pandolfo, Jean Davignon, Nabil G Seidah, Reijo Laaksonen, Gene Set Enrichment Analysis reveals several globally affected pathways due to SKI-1/S1P inhibition in Hep_G2 cells, DNA Cell Biol. 2007 Nov;26(11):765-72. PMID: 17867930.
Eriikka Heikkinen, Teemu Kallonen, Lilli Saarinen, Rolf Sara, Juhani Soini, Audrey King, Frits Mooi, Jussi Mertsola, and Qiushui He, Comparative Genomics of Bordetella pertussis Reveals Progressive Gene Loss in Finnish Strains, PLoS ONE. 2007 Sep 19;2(9):e904.PMID: 17878939
Jaakohuhta S, Härmä H, Tuomola M, Lövgren T. Sensitive Listeria spp. immunoassay based on europium(III) nanoparticulate labels using time-resolved fluorescence. International journal of food microbiology, 2007, 14, 288.
2006
Koskinen JO, Vaarno J, Vainionpaa R, Meltola NJ, Soini AE. A novel separation-free assay technique for serum antibodies using antibody bridging assay principle and two-photon excitation fluorometry. J Immunol Methods. 2006 Feb 20;309(1-2):11-24.
Dimitris Glotsos, Jussi Tohka, Jori Soukka, Juhani Soini and Ulla Ruotsalainen, Detection of outliers and robust estimation of bioaffinity assay fluorescence signals, IEEE Transactions on Information Technology in Biomedicine, (2006) 10 (4), 733-739
Marko E. Tirri, Rina Wahlroos, Niko J. Meltola, Juha Toivonen, Pekka E. Hänninen, Effect of polystyrene microsphere surface to fluorescence lifetime under two-photon excitation, Journal of Fluorescence (2006) 16(6) 809-816.
Pilvi Ylander, Zoltán Bicskei, Pekka Hänninen, Juhani T. Soini Theoretical assessment of errors in rapid immunoassays - how critical is exact timing and reagent concentrations?, Journal of Biophysical Chemistry 123 (2006) 141-145 .
R. Wahlroos, J. Toivonen, M. Tirri and P. Hänninen, Two-photon Excited Fluorescence Energy Transfer. A Study Based on Oligonucleotide rulers., Journal of Fluorescence, (2006), 16, 379-386.
Zoltán Bicskei, Pilvi Ylander, Pekka Hänninen, Calibration of bioaffinity assays using kinetic data, Journal of Biochemical and Biophysical Methods, Volume 67, Issue 1, 30 April 2006, Pages 75-85
2005
M. Tirri, R. J. Huttunen, J. Toivonen, P. L. Härkönen, J. T. Soini, and P. E. Hänninen, Two-photon excitation in fluorescence polarization receptor-ligand binding assay, Journal of Biomolecular Screening 10, 314-319 (2005).
Meltola N, Annales Universitatis Turkuensis AI 339, Dipyrrylmetheneboron difluorides as labels in two-photon excited fluorometry (2005). Doctoral dissertation.
Koskinen JO, Meltola NJ, Soini E and Soini AE. A lab-on-a-chip compatible assay method for human α-fetoprotein. Lab on a Chip (2005). 5(12): 1408-1411.
Meltola NJ, Kettunen MJ and Soini AE. Dipyrrylmetheneboron difluorides as labels in two-photon excited fluorometry. Part I –Immunometric assays. Journal of Fluorescence (2005). 15(3): 231-242.
Meltola NJ, Vaarno J and Soini AE. Dipyrrylmetheneboron difluorides as labels in two-photon excited fluorometry. Part II –Nucleic acid hybridisation assays. Journal of Fluorescence (2005). 15(3): 243-252.
Laihia JK, Koskinen JO, Waris ME, Jansen, CT. Adaptation of the human skin by chronic solar-simulating ultraviolet irradiation prevents ultraviolet-B irradiation –induced rise in serum C-reactive protein levels. Photochem Photobiol. (2005) May-Jun;81(3):654-8.
Soilu-Hänninen M, Koskinen JO, Laaksonen M, Hänninen A, Lilius E.-M, Waris M. High sensitivity measurement of CRP and disease progression in multiple sclerosis. Neurology. (2005) Jul 12;65(1):153-5.
Waris ME, Koskinen JO, Simell O, Knip M, Ilonen J. Onset of beta-cell autoimmunity is not associated with elevated concentration of C-reactive protein in children at genetic risk for Type 1 diabetes. Diabet Med. (2005 Aug;22(8):1123-4.
2004
Meltola, N. J., Wahlroos, R. and Soini, A. E., Hydrophilic labeling reagents of dipyrrylmethene-BF2 dyes for two-photon excited fluorometry: Syntheses and photophysical characterization. Journal of Fluorescence 14, 635-647, (2004).
T. J. Stenholm, J. T. Soini, and P. E. Hänninen, A long-arm autocorrelator for measurement of pico- to nanosecond laser pulse widths, Measurement Science & Technology 15, 2001-2004 (2004).
J. Vaarno, E. Ylikoski, N. J. Meltola, J. T. Soini, P. Hänninen, R. Lahesmaa, and A. E. Soini, New separation-free assay technique for SNPs using two-photon excitation fluorometry, Nucleic Acids Research 32, e108 (2004).
M. Wiklund, J. Toivonen, M. Tirri, P. Hänninen, and H. M. Hertz, Ultrasonic enrichment of microspheres for ultrasensitive biomedical analysis in confocal laser-scanning fluorescence detection, Journal of Applied Physics 96, 1242-1248 (2004).
J. O. Koskinen, J. Vaarno, N. J. Meltola, J. T. Soini, P. E. Hänninen, J. Luotola, M. E. Waris, and A. E. Soini, Fluorescent nanoparticles as labels for immunometric assay of C-reactive protein using two-photon excitation assay technology, Analytical Biochemistry 328, 210-218 (2004).
J. T. Soini, M. E. Waris, and P. E. Hänninen, Detection methods of microsphere based single-step bioaffinity and in vitro diagnostics assays, Journal of Pharmaceutical and Biomedical Analysis 34, 753-760 (2004).
2003
M. Tirri, J. Vaarno, J.T. Soini and P.E. Hänninen, Low cost lasers challenge ultrafast systems in two-photon excitation applications, Opto-electronics review 11(1), 39-44 (2003).